Police are expected to offer further details about the incident at a midday (1000 GMT) press conference.
The 26-year-old born in the United Arab Emirates shouted “Allahu Akbar” (“God is Greatest”) as he fled the scene of the crime in a neighbourhood supermarket before being overpowered by passers-by, witnesses recounted.
The attack had been motivated by “hate,” mayor Olaf Scholz said, although he stopped short of declaring it a terrorist incident.
“These criminals want to poison our free society with fear, but they will not succeed,” he added.
If confirmed as an Islamist attack, it would be the first in Germany since Tunisian Anis Amri drove a truck into crowds at a Berlin Christmas market on December 19th, killing 12 and injuring 48.
But police were unable to immediately confirm the suspect's nationality or identify the motive behind the violence. They have also been unable to confirm the accounts that the man shouted “Allahu Akhbar”.
News website Spiegel Online reported that the individual was named Ahmad A., who arrived in Germany seeking asylum and had contact with the Islamist scene, as well as a history of mental health problems and drug use.
The attacker had been scheduled to be deported, but the process had been held up as he lacked identity papers, Scholz said.
“It makes me especially angry that the perpetrator appears to be a person who claimed protection in Germany and then turned his hate against us,” he added.
Images of heavily-armed police searching the Hamburg accommodation centre where the man lived were posted by newspaper Bild on its website late on Friday.
A police murder unit and a squad specialising in politically-motivated crime are investigating the attack.
Germany has been on high alert over the threat of a jihadist attack since Amri's rampage in Berlin, for which the Islamic State group claimed responsibility.
Jihadists have also carried out a string of random assaults in European countries using knives.
Like the Hamburg attacker, Amri was a failed asylum seeker who could not be deported for lack of documents.
The similarity between the two cases risks reopening barely-healed wounds over Chancellor Angela Merkel's decision to allow more than a million migrants into Germany since 2015, with just two months to go until legislative elections in September.
“Before Mrs Merkel tweets again that this is 'beyond comprehension': this has something to do with Islam. Comprehend that once and for all!” politician Beatrix von Storch of anti-migrant, anti-Islam and anti-European Union party Alternative for Germany (AfD) posted on Twitter.
AfD's support has fallen back in polling since the height of the migrant crisis, but the party remains on course to clear the threshold of five percent of the vote to enter parliament for the first time.
The attacker stabbed to death a 50-year-old man, believed to be a German citizen, and “struck out wildly” at others, wounding a woman and four men aged 19 to 64, police said.
Another 35-year-old man was hurt while overpowering the attacker in the street alongside other passers-by shortly after the killing.
“A crowd of about 30 people ran out of the supermarket. They yelled that someone had been stabbed… we saw a man go past with a big knife, like a butcher's knife, in his hand,” witness Ralf Woyna told AFP.
Woyna had been sitting at a cafe opposite the entrance to the shop where the chase began.
“Two customers who also looked Middle Eastern took all the chairs from the cafe and ran after him. I lost sight of them for a minute and heard a shout of 'Allahu Akbar' in the distance, I knew it was an attack straight away,” he added.
An amateur mobile phone video published by news site Spiegel Online showed a handful of pursuers confronting the attacker, a bearded man wearing a T-shirt and jeans, amid dense city traffic.
They can be seen hurling chairs at him to keep him at a safe distance as he yells and brandishes the knife.
According to Spiegel Online, the 35-year-old man injured during the struggle was the one who finally forced the suspect to the ground, using a pole.
The witnesses slightly hurt the attacker while they were overpowering him, before handing him over to police.
Newspaper Bild published images of the man lying handcuffed on the ground and sitting in the back seat of a police car, a bloodied white bag pulled over his head.