The Las Vegas stalwarts have not performed since the attack in October 2003, when Roy Horn was mauled by one of the pair's famous white tigers in front of a 1,500-strong audience.
But Horn, 64, and partner Siegfried Fischbacher, 69, took to the stage one last time on Saturday night – along with Montecore, the tiger involved in the attack.
In their last appearance at the Bellagio Hotel-Casino the duo appeared on stage in masks and hoods to hide their identities until the last moment.
They used two large glass boxes in their farewell trick. Fischbacher was sealed into one and then both were covered up. When they were uncovered again, Fischbacher had moved into the other box, and the tiger had appeared in the first one.
Neither Siegfried nor Roy spoke during the performance, which was to raise money for charity.